SMARCA2 Rabbit Polyclonal Antibody
cat.: ER65406
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 181 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SMARCA2 aa 231-280 / 1,590. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, HeLa, MCF7, human colon cancer tissue, human prostate cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:100 1:50 1:1,000 |
| Uniprot #: | SwissProt: P51531 Human |
| Alternative names: | ATP dependent helicase SMARCA2 ATP-dependent helicase SMARCA2 BAF190 BAF190B BRG1-associated factor 190B BRM FLJ36757 Global transcription activator homologous sequence hBRM hSNF2a MGC74511 Possible global transcription activator SNF2L2 Probable global transcription activator SNF2L2 Protein brahma homolog SMARCA2 SMCA2_HUMAN SNF2 alpha SNF2 like 2 SNF2-alpha SNF2/SWI2 like protein 2 SNF2L2 SNF2LA Sth1p Sucrose nonfermenting 2 like protein 2 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 |
Images
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Fig1:
Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (ER65406) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 181 kDa Observed band size: 200 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65406) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling SMARCA2 with Rabbit anti-SMARCA2 antibody (ER65406) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SMARCA2 antibody (ER65406) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of MCF7 cells labeling SMARCA2 with Rabbit anti-SMARCA2 antibody (ER65406) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SMARCA2 antibody (ER65406) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SMARCA2 antibody (ER65406) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65406) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-SMARCA2 antibody (ER65406) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65406) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HeLa cells labeling SMARCA2. Cells were fixed and permeabilized. Then stained with the primary antibody (ER65406, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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