FUNDC1 Rabbit Polyclonal Antibody
cat.: ER65664
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N terminal human FUNDC1. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, mouse heart tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue lysate, human liver tissue. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q8IVP5 Human | Q9DB70 Mouse | Q5BJS4 Rat |
| Alternative names: | FUN14 domain containing 1 FUN14 domain containing protein 1 FUN14 domain-containing protein 1 FUND1_HUMAN fundc1 |
Images
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Fig1:
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (ER65664) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: HCT 116 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: Mouse heart tissue lysate (40 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Lane 11: Human liver tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65664) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FUNDC1 antibody (ER65664) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65664) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.