SOCS3 Rabbit Polyclonal Antibody
cat.: ER65765
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human |
| Positive control: | PC-3M cell lysate, mouse spleen tissue lysate, mouse colon tissue lysate, rat spleen tissue lysate, RAW264.7 cell lysate, human colon tissue, mouse spleen tissue, HeLa. |
| Subcellular location: | Cytoplasmic side of plasma membrane |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: O14543 Human |
| Alternative names: | ATOD4 CIS 3 CIS-3 CIS3 Cish3 Cytokine induced SH2 protein 3 Cytokine-inducible SH2 protein 3 E2a Pbx1 target gene in fibroblasts 10 EF 10 MGC71791 SOCS 3 SOCS-3 Socs3 SOCS3_HUMAN SSI 3 SSI-3 SSI3 STAT induced STAT inhibitor 3 STAT-induced STAT inhibitor 3 Suppressor of cytokine signaling 3 |
Images
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Fig1:
Western blot analysis of SOCS3 on different lysates with Rabbit anti-SOCS3 antibody (ER65765) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOCS3 antibody (ER65765) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65765) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-SOCS3 antibody (ER65765) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65765) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling SOCS3. Cells were fixed and permeabilized. Then stained with the primary antibody (ER65765, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.