CCL20/MIP-3 alpha Rabbit Polyclonal Antibody
cat.: ER65786
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CCL20/MIP-3 alpha aa 1-96. |
| Positive control: | Human liver tissue, THP-1. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IF-Cell FC |
1:500 1:500 1:1,000 |
| Uniprot #: | SwissProt: P78556 Human |
| Alternative names: | Beta chemokine exodus 1 Beta-chemokine exodus-1 C C motif chemokine ligand 20 C-C motif chemokine 20 CC chemokine LARC Ccl20 CCL20(2-70) CCL20_HUMAN Chemokine (C C motif) ligand 20 Chemokine C-C motif chemokine 20 Chemokine CC motif ligand 20 CKb4 Exodus 1 Exodus LARC Liver and activation regulated chemokine Liver and activation-regulated chemokine Macrophage inflammatory protein 3 alpha MIP 3 alpha MIP 3A MIP-3-alpha MIP-3a MIP3 alpha MIP3A SCYA20 Small inducible cytokine A20 Small inducible cytokine subfamily A (Cys Cys) member 20 Small-inducible cytokine A20 ST38 MIP-3α MIP 3α |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CCL20/MIP-3 alpha antibody (ER65786) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65786) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunocytochemistry analysis of THP-1 cells labeling CCL20/MIP-3 alpha with Rabbit anti-CCL20/MIP-3 alpha antibody (ER65786) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL20/MIP-3 alpha antibody (ER65786) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of THP-1 cells labeling CCL20/MIP-3 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (ER65786, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.