TIE2 Rabbit Polyclonal Antibody
cat.: ER65864
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 126 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIE2 aa 1-769. |
| Positive control: | EA.hy926 cell lysate, EA.hy926. |
| Subcellular location: | Cell membrane, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q02763 Human |
| Alternative names: | Angiopoietin 1 receptor Angiopoietin-1 receptor CD202b CD202b antigen Endothelial tyrosine kinase Endothelium specific receptor tyrosine kinase 2 hTIE 2 hTIE2 Hyk p140 TEK Soluble TIE2 variant 1 Soluble TIE2 variant 2 Tek tek tyrosine kinase TEK tyrosine kinase endothelial tek tyrosine kinase, endothelial TIE 2 TIE2 TIE2_HUMAN Tunica interna endothelial cell kinase Tyrosine kinase with Ig and EGF homology domains 2 Tyrosine kinase with Ig and EGF homology domains-2 Tyrosine protein kinase receptor TEK Tyrosine protein kinase receptor TIE 2 Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 Venous malformations multiple cutaneous and mucosal VMCM 1 VMCM VMCM1 |
Images
|
Fig1:
Western blot analysis of TIE2 on different lysates with Rabbit anti-TIE2 antibody (ER65864) at 1/2,000 dilution. Lane 1: EA.hy926 cell lysate Lane 2: HEK-293 cell lysate (negative) Lysates/proteins at 15 µg/Lane. Predicted band size: 126 kDa Observed band size: 150 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65864) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of EA.hy926 (positive) and HEK-293 (negative) labeling TIE2 with Rabbit anti-TIE2 antibody (ER65864) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIE2 antibody (ER65864) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of HEK-293 (left, negative) and EA.hy926 (right, positive) cells labeling TIE2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER65864, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.