CD9 Rabbit Polyclonal Antibody
cat.: ER80402
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD9 aa 21-50 / 228. |
| Positive control: | A549, Hela, Jurkat, HepG2, Lovo, human lung cancer tissue, human colon cancer tissue, mouse large intestine tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P21926 Human |
| Alternative names: | Tetraspanin 29 5H9 5H9 antigen Antigen defined by monoclonal 602 29 Antigen defined by monoclonal 60229 BA-2/p24 antigen BA2 BTCC 1 BTCC1 CD9 CD9 antigen CD9 antigen p24 CD9 molecule CD9_HUMAN Cell growth inhibiting gene 2 protein Cell growth-inhibiting gene 2 protein DRAP 27 DRAP27 GIG2 Growth inhibiting gene 2 protein Leukocyte antigen MIC3 MIC3 Motility related protein Motility-related protein MRP 1 MRP-1 MRP1 p24 p24 antigen Tetraspanin-29 Tspan 29 Tspan-29 TSPAN29 |
Images
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Fig1: Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution. |
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Fig2: ICC staining CD9 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD9 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CD9 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-CD9 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Jurkat cells with CD9 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.