CD9 Rabbit Polyclonal Antibody
cat.: ER80402
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD9 aa 196-228 / 228. |
| Positive control: | SW480 cell lysates, Mouse heart tissue lysate, Mouse kidney tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P21926 Human | P40240 Mouse |
| Alternative names: | Tetraspanin 29 5H9 5H9 antigen Antigen defined by monoclonal 602 29 Antigen defined by monoclonal 60229 BA-2/p24 antigen BA2 BTCC 1 BTCC1 CD9 CD9 antigen CD9 antigen p24 CD9 molecule CD9_HUMAN Cell growth inhibiting gene 2 protein Cell growth-inhibiting gene 2 protein DRAP 27 DRAP27 GIG2 Growth inhibiting gene 2 protein Leukocyte antigen MIC3 MIC3 Motility related protein Motility-related protein MRP 1 MRP-1 MRP1 p24 p24 antigen Tetraspanin-29 Tspan 29 Tspan-29 TSPAN29 |
Images
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Fig1:
Western blot analysis of CD9 on SW480 cell lysates with Rabbit anti-CD9 antibody (ER80402) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80402) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ER80402) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80402) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.