Anti-CD9 antibody
cat.: ER80402
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human CD9.
Positive control: A549, Hela, Jurkat, HepG2, Lovo, human lung cancer tissue, human colon cancer tissue, mouse large intestine tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000
1:200
1:200
1:100-1:200
Uniprot #: P21926
Alternative names: Tetraspanin 29 antibody 5H9 antibody 5H9 antigen antibody Antigen defined by monoclonal antibody 602 29 antibody Antigen defined by monoclonal antibody 60229 antibody BA-2/p24 antigen antibody BA2 antibody BTCC 1 antibody BTCC1 antibody CD9 antibody CD9 antigen antibody CD9 antigen p24 antibody CD9 molecule antibody CD9_HUMAN antibody Cell growth inhibiting gene 2 protein antibody Cell growth-inhibiting gene 2 protein antibody DRAP 27 antibody DRAP27 antibody GIG2 antibody Growth inhibiting gene 2 protein antibody Leukocyte antigen MIC3 antibody MIC3 antibody Motility related protein antibody Motility-related protein antibody MRP 1 antibody MRP-1 antibody MRP1 antibody p24 antibody p24 antigen antibody Tetraspanin-29 antibody Tspan 29 antibody Tspan-29 antibody TSPAN29 antibody
Images
ER80402_1.jpg Fig1: Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution.
ER80402_2.jpg Fig2: ICC staining CD9 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER80402_3.jpg Fig3: ICC staining CD9 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER80402_4.jpg Fig4: ICC staining CD9 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER80402_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.
ER80402_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.
ER80402_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-CD9 antibody. Counter stained with hematoxylin.
ER80402_8.jpg Fig8: Flow cytometric analysis of Jurkat cells with CD9 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.