CD3 epsilon Rabbit Polyclonal Antibody
cat.: ER80501
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human CD3 epsilon. |
| Positive control: | Human thymus tissue lysate, Jurkat cell lysateHela, Jurkat, LOVO. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P07766 Human | P22646 Mouse |
| Alternative names: | CD3 epsilon CD3e CD3e antigen epsilon polypeptide (TiT3 complex) CD3E antigen epsilon polypeptide CD3E antigen, epsilon subunit CD3e molecule epsilon CD3e molecule, epsilon (CD3 TCR complex) CD3e molecule, epsilon (CD3-TCR complex) CD3E_HUMAN IMD18 T cell antigen receptor complex epsilon subunit of T3 T cell surface antigen T3/Leu 4 epsilon chain T cell surface glycoprotein CD3 epsilon chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface glycoprotein CD3 epsilon chain T3E TCRE |
Images
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Fig1:
Western blot analysis of CD3E on different lysates using anti-CD3E antibody at 1/1000 dilution. Lane 1: Human thymus tissue lysate Lane 2: Jurkat cell lysate |
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Fig2: ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD3E in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of Jurkat cells with CD3E antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.