CD3 epsilon Rabbit Polyclonal Antibody
cat.: ER80501
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human CD3 epsilon. |
| Positive control: | Jurkat cell lysate, Mouse spleen tissue lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P07766 Human | P22646 Mouse Entrez Gene: 315609 Rat |
| Alternative names: | CD3 epsilon CD3e CD3e antigen epsilon polypeptide (TiT3 complex) CD3E antigen epsilon polypeptide CD3E antigen, epsilon subunit CD3e molecule epsilon CD3e molecule, epsilon (CD3 TCR complex) CD3e molecule, epsilon (CD3-TCR complex) CD3E_HUMAN IMD18 T cell antigen receptor complex epsilon subunit of T3 T cell surface antigen T3/Leu 4 epsilon chain T cell surface glycoprotein CD3 epsilon chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface glycoprotein CD3 epsilon chain T3E TCRE |
Images
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Fig1:
Western blot analysis of CD3 epsilon on different lysates with Rabbit anti-CD3 epsilon antibody (ER80501) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Mouse thymus tissue lysate (40 µg/Lane) Lane 4: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.