Anti-AFP antibody
cat.: ER80601
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 70 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human AFP.
Positive control: NCCIT, HepG2, Jurkat,human liver tissue, human lung tissue, human liver cancer tissue.
Subcellular location: Secreted
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:2,000
1:200
1:200
1:100-1:200
Uniprot #: P02771
Alternative names: Afp antibody AFPD antibody Alpha fetoglobulin antibody Alpha fetoprotein antibody Alpha fetoprotein precursor antibody Alpha-1-fetoprotein antibody Alpha-fetoglobulin antibody Alpha-fetoprotein antibody alpha-fetoprotein, Hereditary persistence of, included antibody FETA antibody FETA_HUMAN antibody Hereditary persistence of alpha fetoprotein antibody HPAFP antibody
Images
ER80601_1.jpg Fig1: Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: human lung
Lane 3: human liver
ER80601_2.jpg Fig2: ICC staining AFP in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER80601_3.jpg Fig3: ICC staining AFP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER80601_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
ER80601_5.jpg Fig5: Flow cytometric analysis of Jurkat cells with AFP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.