Anti-BMP2 antibody
cat.: ER80602
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 75kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Human BMP2 aa 251-300 / 396.
Positive control: MCF-7, Hela, mouse kidney tissue, human kidney tissue, rat kidney tissue, rat small intestine tissue, mouse small intestine tissue, human breast cancer tissue.
Subcellular location: Secreted
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000
1:200
1:200
1:100-1:200
Uniprot #: SwissProt: P12643 Human | P21274 Mouse | P49001 Rat
Alternative names: BDA2 BMP-2 BMP-2A Bmp2 BMP2_HUMAN BMP2A Bone morphogenetic protein 2 Bone morphogenetic protein 2A
Images
ER80602_1.jpg Fig1: Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat kidney
Lane 2: Mouse kidney
Lane 3: Human kidney
Lane 4: Hela
ER80602_2.jpg Fig2: ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
ER80602_3.jpg Fig3: ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
ER80602_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
ER80602_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
ER80602_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
ER80602_7.jpg Fig7: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.