BMP2 Rabbit Polyclonal Antibody
cat.: ER80602
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human BMP2 aa 251-300 / 396. |
| Positive control: | Hela cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, Hela, MCF-7, rat small intestine tissue, human breast cancer tissue, mouse small intestine tissue. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P12643 Human | P21274 Mouse | P49001 Rat |
| Alternative names: | BDA2 BMP-2 BMP-2A Bmp2 BMP2_HUMAN BMP2A Bone morphogenetic protein 2 Bone morphogenetic protein 2A |
Images
|
Fig1:
Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (ER80602) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Lane 2: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80602) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). |
|
Fig3: ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). |
|
Fig4: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin. |
|
Fig7: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.