ET1601-1

LRP1 Recombinant Rabbit Monoclonal Antibody [SA0290]

cat.: ET1601-1

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Tissue, IHC-P, IP, IHC-Fr
Clonality:	Monoclonal
Clone number:	SA0290

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 505 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human LRP1 aa 4,471-4,520 / 4,544.
Positive control:	Mouse liver tissue lysate, Human lung tissue lysate, Rat liver tissue lysate, Rat lung tissue lysate, Mouse lung tissue lysate, Human liver tissue lysate, mouse brain tissue, rat brain tissue, rat liver tissue, human lung tissue, human liver tissue, mouse liver tissue.
Subcellular location:	Cytoplasm, Nucleus, Membrane.
Recommended Dilutions: WB IF-Tissue IHC-P IP IHC-Fr	1:1,000-1:5,000 1:50 1:200-1:2,000 1-2μg/sample 1:100
Uniprot #:	SwissProt: Q07954 Human \| Q91ZX7 Mouse \| G3V928 Rat
Alternative names:	A2MR Alpha 2 macroglobulin receptor alpha 2MR Alpha-2-macroglobulin receptor APOER Apolipoprotein E receptor APR CD 91 CD91 CD91 antigen IGFBP3R LDL receptor related protein 1 Low density lipoprotein receptor related protein 1 Low density lipoprotein related protein 1 Low-density lipoprotein receptor-related protein 1 intracellular domain LRP 1 LRP 515 LRP 85 LRP LRP ICD LRP-1 LRP-515 LRP-85 Lrp1 LRP1 protein LRP1_HUMAN LRP1A LRP515 LRP85 LRPICD MGC88725 Prolow density lipoprotein receptor related protein 1 TbetaR V/LRP 1/IGFBP 3 receptor TbetaRV/LRP1/IGFBP3 receptor TGFBR 5 TGFBR5 Type V tgf beta receptor Low-density lipoprotein receptor-related protein 1 85 kDa subunit

Images

Fig1: Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Human lung tissue lysate
Lane 3: Rat liver tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 505 kDa
Observed band size: 80 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution.

Lane 1: Rat lung tissue lysate
Lane 2: Mouse lung tissue lysate
Lane 3: Human liver tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 505 kDa
Observed band size: 80 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: All lanes: Western blot analysis of LRP1 with anti-LRP1 antibody (ET1601-1) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: LRP1 knockdown Hela whole cell lysate (10 µg). ET1601-1 was shown to specifically react with LRP1 in wild-type Hela cells. Weakened bands were observed when LRP1 knockdown samples were tested. Wild-type and LRP1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunofluorescence analysis of frozen mouse hippocampus tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
	Fig11: Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Fig12: LRP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1601-1 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1601-1 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: ET1601-1 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of ET1601-1 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.