Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP, IHC-Fr |
Clonality: | Monoclonal |
Clone number: | SA0290 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 505 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human LRP1 aa 4,471-4,520 / 4,544. |
Positive control: | Mouse liver tissue lysate, human lung tissue lysate, rat liver tissue lysate, Hela, MCF-7, HUVEC, human lung tissue, mouse brain tissue, mouse liver tissue, human liver tissue, rat lung tissue lysate, mouse lung tissue lysate, human liver tissue lysate. |
Subcellular location: | Cytoplasm, Nucleus, Membrane. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP IHC-Fr |
1:1,000-1:5,000 1:50 1:50 1:200-1:2,000 1:50 Use at an assay dependent concentration. 1:100 |
Uniprot #: | SwissProt: Q07954 Human | Q91ZX7 Mouse | G3V928 Rat |
Alternative names: | A2MR Alpha 2 macroglobulin receptor alpha 2MR Alpha-2-macroglobulin receptor APOER Apolipoprotein E receptor APR CD 91 CD91 CD91 antigen IGFBP3R LDL receptor related protein 1 Low density lipoprotein receptor related protein 1 Low density lipoprotein related protein 1 Low-density lipoprotein receptor-related protein 1 intracellular domain LRP 1 LRP 515 LRP 85 LRP LRP ICD LRP-1 LRP-515 LRP-85 Lrp1 LRP1 protein LRP1_HUMAN LRP1A LRP515 LRP85 LRPICD MGC88725 Prolow density lipoprotein receptor related protein 1 TbetaR V/LRP 1/IGFBP 3 receptor TbetaRV/LRP1/IGFBP3 receptor TGFBR 5 TGFBR5 Type V tgf beta receptor Low-density lipoprotein receptor-related protein 1 85 kDa subunit |
Fig1:
Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Human lung tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
All lanes: Western blot analysis of LRP1 with anti-LRP1 antibody (ET1601-1) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: LRP1 knockdown Hela whole cell lysate (10 µg). ET1601-1 was shown to specifically react with LRP1 in wild-type Hela cells. Weakened bands were observed when LRP1 knockdown samples were tested. Wild-type and LRP1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of LRP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig4: ICC staining of LRP1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig5: ICC staining of LRP1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig6:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig10: Flow cytometric analysis of LRP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-1, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). | |
Fig11:
Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. Lane 1: Rat lung tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig12:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Fig13:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |