ET1601-14

Histone H3 (mono methyl K18) Recombinant Rabbit Monoclonal Antibody [SA42-07]

cat.: ET1601-14

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P
Clonality:	Monoclonal
Clone number:	SA42-07

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 15 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human Histone H3 aa 1-50 / 136 (mono methyl K18) conjugated to Keyhole Limpet Haemocyanin (KLH).
Positive control:	HepG2 cell lysates, mouse testis tissue lysates, HepG2, PC-3M, human colon tissue, mouse testis tissue, mouse kidney tissue.
Subcellular location:	Nucleus, Chromosome
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P	1:500-1:5,000 1:50 1:50 1:50-1:1,000
Uniprot #:	SwissProt: P68431 Human \| P84243 Human \| Q71DI3 Human \| Q16695 Human \| Q6NXT2 Human \| P68433 Mouse \| Q6LED0 Rat
Alternative names:	HIST1 cluster, H3J Histone gene cluster 1, H3 histone family, member E Histone gene cluster 1, H3G H3 histone family, member J HIST1 cluster, H3E HIST1 cluster, H3I Histone gene cluster 1, H3C FLJ92264 H3 histone family, member A H3 histone family, member B H3 histone family, member C H3 histone family, member D H3 histone family, member F H3 histone family, member H H3 histone family, member I H3 histone family, member K H3 histone family, member L H3 histone family, member T H3 histone, family 3A H3.1 H3.3A H3/a H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/l H3/t H31_HUMAN H3F1K H3F3 H3F3A H3FA H3FB H3FC H3FD H3FF H3FH H3FI H3FJ H3FK H3FL HIST1 cluster, H3A HIST1 cluster, H3B HIST1 cluster, H3C HIST1 cluster, H3D HIST1 cluster, H3F HIST1 cluster, H3G HIST1 cluster, H3H HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST......

Images

Fig1: Western blot analysis of Histone H3 (mono methyl K18) on HepG2 cell lysates with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Histone H3 (mono methyl K18) on mouse testis tissue lysates with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: ICC staining Histone H3 (mono methyl K18) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: ICC staining Histone H3 (mono methyl K18) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.

	Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.
	Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.