|Product Type:||Recombinant Rabbit monoclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse, Rat, Zebrafish|
|Applications:||WB, IP, FC|
Western blot analysis of Ras on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: mouse brain tissue lysate
|Fig2: Flow cytometric analysis of Ras was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-16, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).|