Anti-Ras antibody [SA39-05]
cat.: ET1601-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IP, FC
Clonality: Monoclonal
Clone number: SA39-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 18 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Ras aa 20-50.
Positive control: MCF-7 cell lysate, 293T cell lysate, mouse brain tissue lysate, Hela.
Subcellular location: Cytoplasm, Cell membrane, Golgi apparatus.
Recommended Dilutions:

Use at an assay dependent concentration.
Uniprot #: SwissProt: P01111 Human | P01112 Human | P01116 Human | P08556 Mouse | P32883 Mouse | Q61411 Mouse | P08644 Rat | P20171 Rat | Q04970 Rat
Alternative names: C-BAS/HAS antibody c-H-ras antibody C-HA-RAS1 antibody CTLO antibody GTPase HRas antibody GTPase KRas antibody GTPase NRas antibody H-Ras-1 antibody H-RASIDX antibody Ha-Ras antibody HAMSV antibody HRAS antibody HRAS1 antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody K-RAS antibody KRAS antibody KRAS1 antibody KRAS2 antibody N-RAS antibody N-terminally processed antibody NRAS antibody NRAS1 antibody p21ras antibody RASH_HUMAN antibody RASH1 antibody RASK2 antibody Transforming protein p21 antibody v Ha ras Harvey rat sarcoma viral oncogene homolog antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody v ras neuroblastoma RAS viral oncogene homolog antibody
ET1601-16_1.jpg Fig1: Western blot analysis of Ras on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: mouse brain tissue lysate
ET1601-16_2.jpg Fig2: Flow cytometric analysis of Ras was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-16, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).