PBR Recombinant Rabbit Monoclonal Antibody [SA90-03]
cat.: ET1601-19
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SA90-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PBR aa 120-169 / 169.
Positive control: HCT 116 cell lysate, THP-1 cell lysate, 293T cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Hela, PC-3M, SW480, human colon carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue, human prostate carcinoma tissue.
Subcellular location: Mitochondrion membrane
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:2,000
1:50
1:50
1:50-1:400
1:50
Use at an assay dependent concentration.
Uniprot #: SwissProt: P30536 Human | P50637 Mouse
Alternative names: Benzodiazapine receptor (peripheral) Benzodiazepine peripheral binding site BPBS BZRP DBI IBP Isoquinoline carboxamide-binding protein MBR mDRC Mitochondrial benzodiazepine receptor PBR PBS Peripheral benzodiazepine receptor Peripheral benzodiazepine receptor-related protein Peripheral type benzodiazepine receptor Peripheral-type benzodiazepine receptor pk18 PKBS PTBR Ptbzr PTBZR02 RATPTBZR02 translocator protein (18kDa) Translocator protein Tspo Tspo1 TSPOA_HUMAN
Images
ET1601-19_1.jpg Fig1: Western blot analysis of PBR on different lysates with Rabbit anti-PBR antibody (ET1601-19) at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: 293T cell lysate
Lane 4: SK-OV-3 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 19 kDa
Observed band size: 16 kDa

Exposure time: 1 minute 4 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1601-19_2.jpg Fig2: ICC staining of PBR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1601-19_3.jpg Fig3: ICC staining of PBR in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1601-19_4.jpg Fig4: ICC staining of PBR in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1601-19_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-19_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-19_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-19_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-19_9.jpg Fig9: Flow cytometric analysis of PBR was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-19, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
ET1601-19_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PBR antibody (ET1601-19) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-19) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.