ET1601-23

GFAP Recombinant Rabbit Monoclonal Antibody [SA03-04]

cat.: ET1601-23

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IHC-P, IF-Tissue, IHC-Fr, mIHC, IF-Cell
Clonality:	Monoclonal
Clone number:	SA03-04

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 50 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human GFAP aa 1-50 / 432.
Positive control:	Human brain tissue lysate, rat brain tissue lysate, human cerebellum tissue lysate, mouse brain tissue, rat brain tissue, human brain tissue, human glioblastoma tissue, mouse spinal cord tissue, rat spinal cord tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse hippocampus tissue, rat hippocampus tissue.
Subcellular location:	Cytoplasm
Recommended Dilutions: WB IHC-P IF-Tissue IHC-Fr mIHC IF-Cell	1:5,000 1:1,000-1:5,000 1:500-1:1,000 1:500-1:1,000 1:4,000-1:10,000 1:1,000-1:2,000
Uniprot #:	SwissProt: P14136 Human \| P03995 Mouse \| P47819 Rat
Alternative names:	wu:fb34h11 ALXDRD cb345 etID36982.3 FLJ42474 FLJ45472 GFAP GFAP_HUMAN gfapl Glial fibrillary acidic protein Intermediate filament protein wu:fk42c12 xx:af506734 zgc:110485

Images

	Fig1: mIHC analysis of human brain tissue (paraffin-embedded) with GFAP (ET1601-23, 1/4,000) and S100 beta (ET1610-3, 1/2,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
	Fig2: mIHC analysis of mouse hippocampus tissue (paraffin-embedded) with GFAP (ET1601-23, 1/4,000), NeuN (ET1602-12, 1/1,000) and c-Fos (HA722666, 1/200). The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
	Fig3: mIHC analysis of mouse brain tissue (paraffin-embedded) with GFAP (ET1601-23, 1/10,000, Magenta), MAP2 (HA500177, 1/1,000, Red), Olig2 (ET1604-29, 1/5,000, Cyan), and Neun (ET1602-12, 1/10,000, Yellow). The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
	Fig4: mIHC analysis of mouse brain tissue (paraffin-embedded) with GFAP (ET1601-23, 1/5,000, Gray), NeuN (ET1602-12, 1/5,000, red), Iba1 (ET1705-78, 1/2,000, green), Olig2 (ET1604-29, 1/1,000, cyan), anti-MAP2 (HA500177, 1/5,000, magenta) and CD34 (ET1606-11, 1/2,000, yellow) . The immunostaining was performed with the IRISKitCmTSA Kit (900802). DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

	Fig5: Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
	Fig6: Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
	Fig7: Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: C57 brain mixed astrocytes Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ET1601-23, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
	Fig8: Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500

	Fig9: Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/500
	Fig10: Western blot analysis of GFAP on different lysates with Rabbit anti-GFAP antibody (ET1601-23) at 1/5,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-23) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig11: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig12: Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig13: Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig14: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig15: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig16: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1601-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig17: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GFAP antibody (ET1601-23) at 1/500 dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1601-23 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.