LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01]
cat.: ET1601-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SA46-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LAMP2a aa 361-410 / 410. |
| Positive control: | SK-MEL-28 cell lysate, HeLa cell lysate, JAR cell lysate, U-937 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat lung tissue lysate, human kidney tissue, mouse pancreas tissue, rat kidney tissue. |
| Subcellular location: | Cell membrane, Endosome membrane, Lysosome membrane |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P13473 Human | P17047 Mouse | P17046 Rat |
| Alternative names: | CD107 antigen-like family member B CD107b LAMP 2 Lamp 2a LAMP-2 LAMP2 LAMP2_HUMAN Lysosome-associated membrane glycoprotein 2 Lysosome-associated membrane protein 2 |
Images
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Fig1:
Western blot analysis of LAMP2a on different lysates with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: JAR cell lysate (20 µg/Lane) Lane 4: U-937 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (15 µg/Lane) Lane 7: PC-12 cell lysate (15 µg/Lane) Lane 8: Mouse liver tissue lysate (30 µg/Lane) Lane 9: Rat liver tissue lysate (30 µg/Lane) Lane 10: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 45 kDa Observed band size: 70-140 kDa Exposure time: Lane 1-4: 2 minutes 37 seconds; Lane 5-10: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-24) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
LAMP2a was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with ET1601-24 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-24 at 1/2,000 dilution. HRP Conjugated Rabbit IgG kappa light chain antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (Input) Lane 2: ET1601-24 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of ET1601-24 in SK-MEL-28 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.