Anti-LAMP2a antibody [SA46-01]
cat.: ET1601-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SA46-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 120 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human LAMP2a aa 361-410 / 410.
Positive control: Human liver tissue lysate, JAR cell lysate, human placenta tissue lysate, human lung tissue lysate, human liver tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, mouse placenta tissue, mouse pancreas tissue, rat kidney tissue.
Subcellular location: Cell membrane, Endosome membrane, Lysosome membrane
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:5,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P13473 Human | P17047 Mouse | P17046 Rat
Alternative names: CD107 antigen-like family member B CD107b LAMP 2 Lamp 2a LAMP-2 LAMP2 LAMP2_HUMAN Lysosome-associated membrane glycoprotein 2 Lysosome-associated membrane protein 2
Images
ET1601-24_1.jpg Fig1: Western blot analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human liver tissue lysate
Lane 2: JAR cell lysate
Lane 3: human placenta tissue lysate
Lane 4: human lung tissue lysate
ET1601-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-24_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP2a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.