Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04]
cat.: ET1601-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF
Clonality: Monoclonal
Clone number: SA70-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 71 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Alas1 aa 510-640 / 640.
Positive control: HUVEC, MCF-7, Hela, HepG2, JAR.
Subcellular location: Mitochondrion matrix
Recommended Dilutions:
  WB
  ICC/IF

1:1,000-1:5,000
1:50-1:200
Uniprot #: SwissProt: P13196 Human
Alternative names: 5 aminolevulinate synthase 5 aminolevulinate synthase nonspecific mitochondrial 5 aminolevulinic acid synthase 5-aminolevulinate synthase 5-aminolevulinic acid synthase 1 Alas 1 ALAS 3 ALAS ALAS H ALAS HOUSEKEEPING TYPE ALAS N ALAS-H alaS1 ALAS3 ALASH Aminolevulinate delta synthase 1 Aminolevulinic acid synthase 1 Delta ALA synthetase Delta aminolevulinate synthase Delta-ALA synthase 1 Delta-aminolevulinate synthase 1 HEM1_HUMAN MIG 4 MIG4 Migration inducing protein 4 mitochondrial nonspecific
Images
ET1601-26_1.jpg Fig1: Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: JAR
Lane 2: HUVEC
Lane 3: Hela
ET1601-26_2.jpg Fig2: ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1601-26_3.jpg Fig3: ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1601-26_4.jpg Fig4: ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.