Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04]
cat.: ET1601-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SA70-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Alas1 aa 510-640 / 640. |
| Positive control: | JAR cell lysate, HUVEC cell lysate, Hela cell lysate, Hela, HepG2, MCF-7. |
| Subcellular location: | Mitochondrion matrix |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:1,000 1:50 1:50 |
| Uniprot #: | SwissProt: P13196 Human |
| Alternative names: | 5 aminolevulinate synthase 5 aminolevulinate synthase nonspecific mitochondrial 5 aminolevulinic acid synthase 5-aminolevulinate synthase 5-aminolevulinic acid synthase 1 Alas 1 ALAS 3 ALAS ALAS H ALAS HOUSEKEEPING TYPE ALAS N ALAS-H alaS1 ALAS3 ALASH Aminolevulinate delta synthase 1 Aminolevulinic acid synthase 1 Delta ALA synthetase Delta aminolevulinate synthase Delta-ALA synthase 1 Delta-aminolevulinate synthase 1 HEM1_HUMAN MIG 4 MIG4 Migration inducing protein 4 mitochondrial nonspecific |
Images
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Fig1:
Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Alas1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/500 dilution. Lane 1: JAR cell lysate Lane 2: HUVEC cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of HepG2 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of MCF-7 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.