RPS3 Recombinant Rabbit Monoclonal Antibody [SA46-08]
cat.: ET1601-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SA46-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 27 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RPS3 aa 125-174 / 243.
Positive control: Jurkat cell lysate, PC-12 cell lysate, human kidney tissue, mouse lung tissue.
Subcellular location: Cytoplasm, Nucleus, Mitochondrion inner membrane
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: P23396 Human | P62908 Mouse | P62909 Rat
Alternative names: 40S ribosomal protein S3 fb13d09 FLJ26283 FLJ27450 IMR 90 ribosomal protein S3 MGC56088 MGC87870 OTTHUMP00000229804 OTTHUMP00000229805 OTTHUMP00000229874 OTTHUMP00000229877 OTTHUMP00000229878 OTTHUMP00000229879 OTTHUMP00000229880 OTTHUMP00000229882 OTTHUMP00000229883 OTTHUMP00000229886 Ribosomal protein S3 rps3 RS3_HUMAN S3 wu:fb13d09 zgc:56088
Images
ET1601-27_1.jpg Fig1: Western blot analysis of RPS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate
ET1601-27_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1601-27_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-27, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.