RPS3 Recombinant Rabbit Monoclonal Antibody [SA46-08]
cat.: ET1601-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SA46-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPS3 aa 125-174 / 243. |
| Positive control: | Jurkat cell lysate, PC-12 cell lysate, human kidney tissue, mouse lung tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion inner membrane |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P23396 Human | P62908 Mouse | P62909 Rat |
| Alternative names: | 40S ribosomal protein S3 fb13d09 FLJ26283 FLJ27450 IMR 90 ribosomal protein S3 MGC56088 MGC87870 OTTHUMP00000229804 OTTHUMP00000229805 OTTHUMP00000229874 OTTHUMP00000229877 OTTHUMP00000229878 OTTHUMP00000229879 OTTHUMP00000229880 OTTHUMP00000229882 OTTHUMP00000229883 OTTHUMP00000229886 Ribosomal protein S3 rps3 RS3_HUMAN S3 wu:fb13d09 zgc:56088 |
Images
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Fig1:
Western blot analysis of RPS3 on different lysates with Rabbit anti-RPS3 antibody (ET1601-27) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-27) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-27, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.