JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]
cat.: ET1601-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cow, Monkey, Zebrafish |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SA43-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48/53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human JNK1 aa 1-423 / 427. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, NIH/3T3, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane, Mitochondrion |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:2,000 1:50 1:50 1:400-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P45983 Human | P45984 Human | P53779 Human | Q61831 Mouse | Q91Y86 Mouse | Q9WTU6 Mouse | P49185 Rat | P49186 Rat | P49187 Rat |
| Alternative names: | C Jun kinase 2 c Jun N terminal kinase 1 c Jun N terminal kinase 2 c Jun N terminal kinase 3 c-Jun N-terminal kinase 1 JNK 46 JNK 55 JNK JNK-46 JNK1 JNK1A2 JNK2 JNK21B1/2 JNK2A JNK2ALPHA JNK2B JNK2BETA JNK3 alpha protein kinase JNK3 JNK3A Jun kinase JUN N terminal kinase MAP kinase 10 MAP kinase 8 MAP kinase 9 MAP kinase p49 3F12 MAPK 10 MAPK 8 MAPK 9 MAPK10 mapk8 MAPK9 Mitogen activated protein kinase 10 Mitogen activated protein kinase 8 Mitogen activated protein kinase 8 isoform JNK1 alpha1 Mitogen activated protein kinase 8 isoform JNK1 beta2 Mitogen activated protein kinase 9 Mitogen-activated protein kinase 8 MK08_HUMAN p493F12 p54a p54aSAPK p54bSAPK PRKM10 PRKM8 PRKM9 SAPK SAPK(beta) SAPK1 SAPK1a SAPK1b SAPK1c Stress activated protein kinase 1 Stress activated protein kinase 1a Stress activated protein kinase 1b Stress activated protein kinase 1c Stress activated prote...... |
Images
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Fig1:
Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of JNK1+JNK2+JNK3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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