ET1601-28

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cow, Monkey, Zebrafish
Applications:	WB, ICC/IF, IP
Clonality:	Monoclonal
Clone number:	SA43-06

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1 mg/mL.

Purification:

Protein A affinity purified.

Molecular weight:

46/54 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within human JNK1 aa 1-400.

Positive control:

Hela cell lysate, PC-12 cell lysate, K562 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, NIH/3T3.

Subcellular location:

Cytoplasm, Nucleus, Membrane, Mitochondrion

Recommended Dilutions:
  WB
  ICC/IF
  IP

1:500-1:5,000
1:50-1:200
Use at an assay dependent concentration.

Uniprot #:

Alternative names:

C Jun kinase 2 antibody c Jun N terminal kinase 1 antibody c Jun N terminal kinase 2 antibody c Jun N terminal kinase 3 antibody c-Jun N-terminal kinase 1 antibody JNK 46 antibody JNK 55 antibody JNK antibody JNK-46 antibody JNK1 antibody JNK1A2 antibody JNK2 antibody JNK21B1/2 antibody JNK2A antibody JNK2ALPHA antibody JNK2B antibody JNK2BETA antibody JNK3 alpha protein kinase antibody JNK3 antibody JNK3A antibody Jun kinase antibody JUN N terminal kinase antibody MAP kinase 10 antibody MAP kinase 8 antibody MAP kinase 9 antibody MAP kinase p49 3F12 antibody MAPK 10 antibody MAPK 8 antibody MAPK 9 antibody MAPK10 antibody mapk8 antibody MAPK9 antibody Mitogen activated protein kinase 10 antibody Mitogen activated protein kinase 8 antibody Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody Mitogen activated protein kinase 9 antibody Mitogen-activated protein kinase 8 antibody MK08_HUMAN antibody p493F12 antibody p54a antibody p54aSAPK antibody p54bSAPK antibody PRKM10 antibody PRKM8 antibody PRKM9 antibody SAPK antibody SAPK(beta) antibody SAPK1 antibody SAPK1a antibody SAPK1b antibody SAPK1c antibody Stress activated protein kinase 1 antibody Stress activated protein kinase 1a antibody Stress activated protein kinase 1b antibody Stress activated protein kinase 1c antibody Stress activated protein kinase beta antibody Stress activated protein kinase JNK1 antibody Stress activated protein kinase JNK2 antibody Stress activated protein kinase JNK3 antibody Stress-activated protein kinase 1c antibody Stress-activated protein kinase JNK1 antibody

	Fig1: Western blot analysis of JNK1+JNK2+JNK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 2: K562 cell lysate
	Fig2: Western blot analysis of JNK1+JNK2+JNK3 on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
	Fig3: ICC staining of JNK1+JNK2+JNK3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).