Integrin beta 1 Recombinant Rabbit Monoclonal Antibody [SR30-03]
cat.: ET1602-10
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SR30-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin beta-1 aa 652-701 / 798. |
| Positive control: | HeLa cell lysate, A431 cell lysate, human skin tissue, mouse skin tissue, rat skin tissue. |
| Subcellular location: | Cell membrane, Cell projection, Cleavage furrow |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:200-1:1,000 1:100 |
| Uniprot #: | SwissProt: P05556 Human | P09055 Mouse | P49134 Rat |
| Alternative names: | beta1 integrin CD29 Fibronectin receptor subunit beta FNRB Glycoprotein IIa GP IIa GPIIA Integrin beta-1 integrin VLA-4 beta subunit Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) ITB1_HUMAN ITGB1 MDF2 MSK12 OTTHUMP00000019420 Very late activation protein, beta polypeptide VLA BETA VLA-4 subunit beta VLA-BETA VLAB VLAbeta |
Images
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Fig1:
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 88 kDa Observed band size: 120-140 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-10) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Integrin beta 1 with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1602-10, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.