Anti-NeuN antibody [SR45-07]
cat.: ET1602-12
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: SR45-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 34 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human NeuN aa 20-60.
Positive control: Human brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate, SH-SY5Y, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, human brain tissue.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:200-1:500
1:50-1:100
Uniprot #: SwissProt: A6NFN3 Human | Q8BIF2 Mouse
Unigene: 143966 Rat
Alternative names: FLJ56884 antibody FLJ58356 antibody Fox-1 homolog C antibody fox1 homolog C antibody Fox3 antibody FOX3NeuN antibody hexaribonucleotide binding protein 3 antibody HRNBP3 antibody NEUN antibody neuronal nuclei antibody Rbfox3 antibody RFOX3_HUMAN antibody RNA binding protein fox-1 homolog 3 antibody RNA binding protein, fox 1 homolog (C. elegans) 3 antibody hide
Images
ET1602-12_1.jpg Fig1: Western blot analysis of NeuN on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human brain tissue lysate
Lane 2: rat brain tissue lysate
Lane 2: mouse brain tissue lysate
ET1602-12_2.jpg Fig2: ICC staining of NeuN in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-12, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-12_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-12_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-12_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-12_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-12_7.jpg Fig7: Flow cytometric analysis of NeuN was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-12, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.