Phospho-Glycogen synthase 1 (S641) Recombinant Rabbit Monoclonal Antibody [SR46-06]
cat.: ET1602-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SR46-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser641 of Human Glycogen synthase 1. |
| Positive control: | SK-Br-3 cell lysate, A431 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, A549, NIH/3T3, mouse liver tissue, mouse skeletal muscle tissue, mouse smooth muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:500 1:50 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P13807 Human | Q9Z1E4 Mouse Entrez Gene: 690987 Rat |
| Alternative names: | Glycogen [starch] synthase Glycogen synthase 1 (muscle) Glycogen synthase 1 GSY GYS Gys1 GYS1_HUMAN muscle |
Images
|
Fig1:
Western blot analysis of Phospho-Glycogen synthase 1 (S641) on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 (S641) antibody (ET1602-13) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 1 minute 34 seconds |
|
Fig2: ICC staining of Phospho-Glycogen synthase 1 (S641) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-13, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of Phospho-Glycogen synthase 1 (S641) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-13, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1 (S641) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1 (S641) antibody (ET1602-13) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1 (S641) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1 (S641) antibody (ET1602-13) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Western blot analysis of Phospho-Glycogen synthase 1 (S641) on different lysates with Rabbit anti-Phospho-Glycogen synthase 1 (S641) antibody (ET1602-13) at 1/1,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-13, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 83.8 kDa Observed band size: 83.8 kDa |
|
Fig9:
Western blot analysis of Phospho-Glycogen synthase 1 (S641) on different lysates with Rabbit anti-Phospho-Glycogen synthase 1 (S641) antibody (ET1602-13) at 1/1,000 dilution. Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-13, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 83.8 kDa Observed band size: 83.8 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.