Collagen XVII Recombinant Rabbit Monoclonal Antibody [SR46-05]
cat.: ET1602-14
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: SR46-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 148 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Collagen XVII aa 1313-1420 / 1497.
Positive control: A431 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, HUVEC, human skin tissue, mouse skin tissue, rat skin tissue.
Subcellular location: Cell junction, Membrane, Secreted
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
1:1,000-1:5,000
1:50
1:50
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9UMD9 Human | Q07563 Mouse
Unigene: 40392 Rat
Alternative names: 180 kDa bullous pemphigoid antigen 2 Alpha 1 type XVII collagen BA16H23.2 BP 180 BP180 BPA 2 BPAG 2 BPAG2 Bullous pemphigoid antigen 2 COL17A1 Collagen 17 Collagen alpha 1 XVII chain Collagen alpha 1(XVII) chain Collagen alpha1 XVII chain Collagen type XVII alpha 1 Collagen XVII alpha 1 polypeptide CollagenXVII Epidermolysis bullosa junctional localisata variant FLJ60881 KIAA0204 LAD 1 LAD1
Images
ET1602-14_1.jpg Fig1: Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/5,000 dilution.

Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: Mouse skin tissue lysate (20 µg/Lane)
Lane 3: Rat skin tissue lysate (20 µg/Lane)

Predicted band size: 148 kDa
Observed band size: 130、180 kDa

Exposure time: 40 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1602-14_2.jpg Fig2: ICC staining of Collagen XVII in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-14, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-14_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-14_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-14_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Collagen XVII antibody (ET1602-14) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.