Rab4A Recombinant Rabbit Monoclonal Antibody [SR27-08]
cat.: ET1602-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Monkey, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | SR27-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Rab4A aa 169-218 / 218. |
| Positive control: | MCF-7 cell lysate, 293T cell lysate, COS-1 cell lysate, PC-12. |
| Subcellular location: | Cytoplasm, Membrane |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP |
1:500 1:50 1:50 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P20338 Human | P56371 Mouse | P05714 Rat |
| Alternative names: | HRES 1 / RAB4 Oncogene RAB4 Rab 4 RAB 4A RAB4 member RAS oncogene family Rab4a RAB4A member RAS oncogene family RAB4A_HUMAN Ras related protein Rab 4A Ras related protein Rab4A Ras-related protein Rab-4A |
Images
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Fig1:
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate Lane 3: COS-1 cell lysate |
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Fig2: ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.