Rab4A Recombinant Rabbit Monoclonal Antibody [SR27-08]
cat.: ET1602-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Monkey, Mouse
Applications: WB, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: SR27-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Rab4A aa 169-218 / 218.
Positive control: MCF-7 cell lysate, 293T cell lysate, COS-1 cell lysate, PC-12.
Subcellular location: Cytoplasm, Membrane
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP

1:500
1:50
1:50
Use at an assay dependent concentration.
Uniprot #: SwissProt: P20338 Human | P56371 Mouse | P05714 Rat
Alternative names: HRES 1 / RAB4 Oncogene RAB4 Rab 4 RAB 4A RAB4 member RAS oncogene family Rab4a RAB4A member RAS oncogene family RAB4A_HUMAN Ras related protein Rab 4A Ras related protein Rab4A Ras-related protein Rab-4A
Images
ET1602-15_1.jpg Fig1: Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate
ET1602-15_2.jpg Fig2: ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.