Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01]
cat.: ET1602-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SR39-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cdk5 aa 243-292 / 292. |
| Positive control: | HCT 116 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human thyroid carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:400 |
| Uniprot #: | SwissProt: Q00535 Human | P49615 Mouse | Q03114 Rat |
| Alternative names: | Cdk 5 Cdk5 CDK5_HUMAN Cell division protein kinase 5 Crk6 Cyclin dependent kinase 5 Cyclin-dependent kinase 5 Protein kinase CDK5 splicing PSSALRE Serine threonine protein kinase PSSALRE Serine/threonine-protein kinase PSSALRE Tau protein kinase II catalytic subunit TPKII catalytic subunit |
Images
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Fig1:
Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/500 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Cdk5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-17) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.