ET1602-17

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01]

cat.: ET1602-17

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC, IP
Clonality:	Monoclonal
Clone number:	SR39-01

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 33 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human Cdk5 aa 243-292 / 292.
Positive control:	Hela cell lysate, SH-SY5Y cell lysate, MCF-7 cell lysate, rat brain tissue, mouse brain tissue, human thyroid carcinoma tissue, Hela.
Subcellular location:	Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction
Recommended Dilutions: WB IHC-P FC IP	1:500 1:50-1:400 1:50 Use at an assay dependent concentration.
Uniprot #:	SwissProt: Q00535 Human \| P49615 Mouse \| Q03114 Rat
Alternative names:	Cdk 5 Cdk5 CDK5_HUMAN Cell division protein kinase 5 Crk6 Cyclin dependent kinase 5 Cyclin-dependent kinase 5 Protein kinase CDK5 splicing PSSALRE Serine threonine protein kinase PSSALRE Serine/threonine-protein kinase PSSALRE Tau protein kinase II catalytic subunit TPKII catalytic subunit

Images

	Fig1: Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/500 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Cdk5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-17) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: MCF-7 cell lysate
	Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdk5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cdk5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Flow cytometric analysis of Cdk5 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-17, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.