Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
Clonality: | Monoclonal |
Clone number: | SR41-02 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 68 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human SHP1 aa 537-595 / 595. |
Positive control: | Jurkat cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat thymus tissue lysate, Hela, HepG2, rat brain tissue, rat spleen tissue, human tonsil tissue, mouse liver tissue, mouse brain tissue, mouse spleen tissue. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:5,000 1:50 1:50 1:200-1:1,000 1:50 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P29350 Human | P29351 Mouse | P81718 Rat |
Alternative names: | 70Z-SHP EC 3.1.3.48 HCP HCPH Hematopoietic cell phosphatase Hematopoietic cell protein tyrosine phosphatase Hematopoietic cell protein-tyrosine phosphatase HPTP1C Protein tyrosine phosphatase 1C Protein tyrosine phosphatase non receptor type 6 Protein tyrosine phosphatase SHP1 Protein-tyrosine phosphatase 1C protein-tyrosine phosphatase SHP 1 Protein-tyrosine phosphatase SHP-1 PTN6_HUMAN PTP 1C PTP-1C PTP1C Ptpn6 SH PTP 1 SH PTP1 SH-PTP1 SHP 1 SHP 1L SHP1 SHP1L tyrosine protein phosphatase non receptor type 6 Tyrosine-protein phosphatase non-receptor type 6 |
Fig1:
Western blot analysis of SHP1 on different lysates with Rabbit anti-SHP1 antibody (ET1602-19) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: RAW264.7 cell lysate (15 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat thymus tissue lysate (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 24 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-19) at 1/5,000dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of SHP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: ICC staining of SHP1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig9: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SHP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-19, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig10: Flow cytometric analysis of SHP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-19, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |