Anti-Phospho-Histone H2A.X(S139) antibody [SR33-09]
cat.: ET1602-2
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SR33-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 15 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Positive control: HepG2 cell lysate–treated with etoposide, HepG2 cell lysate, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue.
Subcellular location: Nucleus, Chromosome
Recommended Dilutions:
  WB
  IHC-P

1:500-1:5,000
1:50-1:200
Uniprot #: SwissProt: P16104 Human | P27661 Mouse
Unigene: 2850 Rat
Alternative names: AW228881 H2A histone family member X H2A.FX H2A.X H2a/x H2AFX H2AX H2AX histone H2AX_HUMAN Hist5.2ax Histone 2A Histone 2AX Histone H2A.X Histone H2AX RGD1566119
Images
ET1602-2_1.jpg Fig1: Western blot analysis of Phospho-Histone H2A.X(S139) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate–treated with etoposide
Lane 2: HepG2 cell lysate–untreated
ET1602-2_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-Histone H2A.X(S139) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-2_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-Histone H2A.X(S139) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-2_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Histone H2A.X(S139) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-2_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Phospho-Histone H2A.X(S139) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.