ET1602-20

PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09]

cat.: ET1602-20

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Tissue, IHC-P, IP, IHC-Fr
Clonality:	Monoclonal
Clone number:	SR38-09

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 80 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human PSD95 aa 1-40.
Positive control:	Human brain tissue lysate, mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue, mouse cerebellum tissue.
Subcellular location:	Cell membrane, Postsynaptic density, Synapse, Cytoplasm, Cell projection, axon, dendritic spine, dendrite, Presynapse.
Recommended Dilutions: WB IF-Tissue IHC-P IP IHC-Fr	1:2,000 1:500 1:2,000 Use at an assay dependent concentration. 1:500
Uniprot #:	SwissProt: P78352 Human \| Q62108 Mouse \| P31016 Rat
Alternative names:	Discs large homolog 4 Disks large homolog 4 DLG 4 Dlg4 DLG4_HUMAN FLJ97752 FLJ98574 Human post synaptic density protein 95 Post synaptic density protein 95 Postsynaptic density protein 95 PSD 95 PSD-95 PSD95 SAP 90 SAP-90 SAP90 Synapse associated protein 90 Synapse-associated protein 90 Tax interaction protein 15

Images

Fig1: Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/500 dilution.

Important Notice: Antigen retrieval is not required before IHC-Fr staining.

The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1602-20, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Chicken anti-Iba1 antibody (HA601376, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 was used as the secondary antibody at 1/1,000 dilution.

Fig2: Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse hippocampus tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat hippocampus tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 80 kDa
Observed band size: 75-100 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling PSD95 with Rabbit anti-PSD95 antibody (ET1602-20) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1602-20, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.