Anti-PSD95 antibody [SR38-09]
cat.: ET1602-20
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SR38-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 90 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PSD95 aa 1-40.
Positive control: Mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, N2A, SH-SY5Y, rat brain tissue, mouse cerebullum tissue.
Subcellular location: Cell membrane, Cell junction
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P78352 Human | Q62108 Mouse | P31016 Rat
Alternative names: Discs large homolog 4 antibody Disks large homolog 4 antibody DLG 4 antibody Dlg4 antibody DLG4_HUMAN antibody FLJ97752 antibody FLJ98574 antibody Human post synaptic density protein 95 antibody Post synaptic density protein 95 antibody Postsynaptic density protein 95 antibody PSD 95 antibody PSD-95 antibody PSD95 antibody SAP 90 antibody SAP-90 antibody SAP90 antibody Synapse associated protein 90 antibody Synapse-associated protein 90 antibody Tax interaction protein 15 antibody
Images
ET1602-20_1.jpg Fig1: Western blot analysis of PSD95 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse brain tissue lysate
Lane 2: human brain tissue lysate
Lane 3: rat brain tissue lysate
ET1602-20_2.jpg Fig2: ICC staining of PSD95 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-20_3.jpg Fig3: ICC staining of PSD95 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-20_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-20_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-20_6.jpg Fig6: Flow cytometric analysis of PSD95 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-20, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.