MERTK Recombinant Rabbit Monoclonal Antibody [SR29-07]
cat.: ET1602-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SR29-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MERTK aa 1-118 / 999. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, human tonsil tissue, 293T. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC IF-Tissue |
1:1,000 1:50-1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q12866 Human |
| Alternative names: | c MER c mer proto oncogene tyrosine kinase c-mer cMER cmer protooncogene tyrosine kinase Eyk MER MER receptor tyrosine kinase MERK MERPEN Mertk MERTK c-mer proto-oncogene tyrosine kinase MERTK_HUMAN MGC133349 nmf12 Nyk Proto oncogene tyrosine protein kinase MER Proto oncogene tyrosine protein kinase MER precursor Proto-oncogene c-Mer Receptor tyrosine kinase MerTK RP38 STK kinase Tyrosine-protein kinase Mer |
Images
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Fig1:
Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (ET1602-21) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (ET1602-21) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MERTK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-21) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MERTK antibody (ET1602-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling MERTK with Rabbit anti-MERTK antibody (ET1602-21) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1602-21, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Flow cytometric analysis of 293T cells labeling MERTK. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1602-21, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.