Rho A+B+C Recombinant Rabbit Monoclonal Antibody [SR38-00]
cat.: ET1602-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SR38-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RhoC aa aa 1-193 / 193. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, HeLa, NIH/3T3, C6. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Cleavage furrow. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:100 1:1,000 |
| Uniprot #: | SwissProt: P08134 Human | P61586 Human | P62745 Human | P62746 Mouse | Q62159 Mouse | Q9QUI0 Mouse | P61589 Rat | P62747 Rat |
| Alternative names: | AA017882 AI324259, Aplysia ras related homolog 9 (RhoC) Aplysia ras-related homolog 12 Aplysia RAS-related homolog 6 Aplysia RAS-related homolog ARH12 ARH6 ARH9 ARHA ARHB ARHC H12 H6 H9 MGC117867 MGC1448 MGC61427 MST081 MSTP081 Oncogene RHO H12 Oncogene RHO H6 Oncogene RHO H9 OTTHUMP00000013675 OTTHUMP00000013676 OTTHUMP00000013802 OTTHUMP00000013805 OTTHUMP00000013807 OTTHUMP00000013809 OTTMUSP00000025086 OTTMUSP00000025087 Ras homolog 9 (RhoC) Ras homolog gene family, member A Ras homolog gene family, member B Ras homolog gene family, member C RAS-related homolog 9 Rh -related GTP binding protein RhoC Rho cDNA clone 12 rho cDNA clone 6 rho cDNA clone 9 Rho related GTP binding protein RhoB Rho related GTP binding protein RhoC RHO12 RHOA RHOB RHOC rhoC GTPase RHOH12 RHOH6 RHOH9 RP11-426L16.4 small GTP binding protein RhoA Small GTP binding protein RhoC Transforming protein RhoA |
Images
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Fig1:
Western blot analysis of Rho A+B+C on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling Rho A+B+C with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling Rho A+B+C with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of C6 cells labeling Rho A+B+C with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rho A+B+C antibody (ET1602-23) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling Rho A+B+C. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-23, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of NIH/3T3 cells labeling Rho A+B+C. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-23, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7:
Flow cytometric analysis of C6 cells labeling Rho A+B+C. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-23, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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