Anti-p38 antibody [SR43-04]
cat.: ET1602-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF
Clonality: Monoclonal
Clone number: SR43-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human p38 aa 160-200.
Positive control: Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Jurkat cell lysate, Hela, NIH/3T3.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC/IF

1:1,000-1:5,000
1:50-1:200
Uniprot #: SwissProt: Q16539 Human | P47811 Mouse | P70618 Rat
Alternative names: CSAID Binding Protein 1 antibody CSAID binding protein antibody CSAID-binding protein antibody Csaids binding protein antibody CSBP 1 antibody CSBP 2 antibody CSBP antibody CSBP1 antibody CSBP2 antibody CSPB1 antibody Cytokine suppressive anti-inflammatory drug-binding protein antibody EXIP antibody MAP kinase 14 antibody MAP kinase MXI2 antibody MAP kinase p38 alpha antibody MAPK 14 antibody MAPK14 antibody MAX interacting protein 2 antibody MAX-interacting protein 2 antibody Mitogen Activated Protein Kinase 14 antibody Mitogen activated protein kinase p38 alpha antibody Mitogen-activated protein kinase 14 antibody Mitogen-activated protein kinase p38 alpha antibody MK14_HUMAN antibody Mxi 2 antibody MXI2 antibody p38 ALPHA antibody p38 antibody p38 MAP kinase antibody p38 MAPK antibody p38 mitogen activated protein kinase antibody p38ALPHA antibody p38alpha Exip antibody PRKM14 antibody PRKM15 antibody RK antibody SAPK2A antibody
Images
ET1602-26_1.jpg Fig1: Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Jurkat cell lysate
ET1602-26_2.jpg Fig2: ICC staining of p38 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-26_3.jpg Fig3: ICC staining of p38 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.