p38 alpha/MAPK14 Recombinant Rabbit Monoclonal Antibody [SR43-04]
cat.: ET1602-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | SR43-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human p38 aa 160-200. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, THP-1 cell lysate, Jurkat cell lysate, C2C12 cell lysate, rat kidney tissue lysate, mouse kidney tissue lysate, HeLa, RAW264.7, C6. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC |
1:1,000 1:100-1:500 1:50 1:1,000 |
| Uniprot #: | SwissProt: Q16539 Human | P47811 Mouse | P70618 Rat |
| Alternative names: | CSAID Binding Protein 1 CSAID binding protein CSAID-binding protein Csaids binding protein CSBP 1 CSBP 2 CSBP CSBP1 CSBP2 CSPB1 Cytokine suppressive anti-inflammatory drug-binding protein EXIP MAP kinase 14 MAP kinase MXI2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX interacting protein 2 MAX-interacting protein 2 Mitogen Activated Protein Kinase 14 Mitogen activated protein kinase p38 alpha Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 alpha MK14_HUMAN Mxi 2 MXI2 p38 ALPHA p38 p38 MAP kinase p38 MAPK p38 mitogen activated protein kinase p38ALPHA p38alpha Exip PRKM14 PRKM15 RK SAPK2A |
Images
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Fig1:
Western blot analysis of p38 alpha/MAPK14 on different lysates with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lane 4: THP-1 cell lysate Lane 5: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of p38 alpha/MAPK14 on different lysates with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: C2C12 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of p38 alpha/MAPK14 on different lysates with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD p38 alpha/MAPK14 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of HeLa cells treated with UV for 30 minutes then recover 30 minutes labeling p38 alpha/MAPK14 with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of RAW264.7 cells labeling p38 alpha/MAPK14 with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Immunocytochemistry analysis of C6 cells labeling p38 alpha/MAPK14 with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha/MAPK14 antibody (ET1602-26) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig7:
Flow cytometric analysis of HeLa cells labeling p38 alpha/MAPK14. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-26, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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