PI 3 Kinase p55 gamma Recombinant Rabbit Monoclonal Antibody [SR44-06]
cat.: ET1602-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SR44-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PI 3 Kinase p55 gamma aa 412-461 / 461. |
| Positive control: | MCF-7 cell lysate, Jurkat cell lysate, human tonsil tissue lysate, PC-3M, SH-SY5Y, mouse testis tissue, mouse brain tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500 1:50 1:50 1:200 |
| Uniprot #: | SwissProt: Q92569 Human | Q64143 Mouse | Q63789 Rat |
| Alternative names: | DKFZp686P05226 FLJ41892 OTTHUMP00000009783 OTTHUMP00000009786 p55 p55 gamma P55G_HUMAN p55PIK Phosphatidylinositol 3 kinase regulatory subunit gamma Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma Phosphatidylinositol 3-kinase regulatory subunit gamma Phosphoinositide 3 kinase regulatory subunit 3 (gamma) Phosphoinositide 3 kinase regulatory subunit 3 Phosphoinositide 3 kinase regulatory subunit polypeptide 3 Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) PI3 kinase p85 subunit gamma PI3-kinase regulatory subunit gamma PI3-kinase subunit p55-gamma PI3K regulatory subunit gamma Pik3r3 PtdIns 3 kinase p85 gamma PtdIns-3-kinase regulatory subunit gamma PtdIns-3-kinase regulatory subunit p55-gamma |
Images
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Fig1:
Western blot analysis of PI 3 Kinase p55 gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 3: human tonsil tissue lysate |
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Fig2: ICC staining of PI 3 Kinase p55 gamma in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of PI 3 Kinase p55 gamma in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-27, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-27, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.