Caspase-2 Recombinant Rabbit Monoclonal Antibody [SR44-01]
cat.: ET1602-30
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: SR44-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse Caspase-2 aa 294-452 / 452.
Positive control: 293 cell lysate, Jurkat cell lysate, HUVEC cell lysate, human lung carcinoma tissue lysate, Hela, A549, human kidney tissue, mouse kidney tissue.
Subcellular location: Cytoplasm, Nucleus, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500
1:50
1:50
1:50
1:50
Uniprot #: SwissProt: P42575 Human | P29594 Mouse
Alternative names: Caspase2 CASP 2 CASP-2 Casp2 CASP2_HUMAN Caspase 2 Caspase 2 apoptosis related cysteine peptidase Caspase-2 subunit p12 Caspase2 ICH 1 ICH 1 protease ICH 1L ICH1 ICH1 protease ICH1L NEDD-2 NEDD2 Neural precursor cell expressed developmentally down-regulated protein 2 PPP1R57 Protease ICH-1 Protein phosphatase 1 regulatory subunit 57
Images
ET1602-30_1.jpg Fig1: Western blot analysis of Caspase-2 on different lysates with Rabbit anti-Caspase-2 antibody (ET1602-30) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Caspase-2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 1 minute 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-30) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1602-30_2.jpg Fig2: Western blot analysis of Caspase-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 2: 293 cell lysate
Lane 2: Jurkat cell lysate
Lane 2: HUVEC cell lysate
Lane 1: human lung carcinoma tissue lysate
ET1602-30_3.jpg Fig3: ICC staining of Caspase-2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-30_4.jpg Fig4: ICC staining of Caspase-2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-30_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caspase-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-30_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-30_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-30_8.jpg Fig8: Flow cytometric analysis of Caspase-2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-30, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.