Scavenging Receptor SR-BI Recombinant Rabbit Monoclonal Antibody [SR37-06]
cat.: ET1602-32
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: SR37-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SRB1 aa 81-130 / 552.
Positive control: HepG2 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, CRC, PC-12, human liver tissue, human spleen tissue, mouse liver tissue.
Subcellular location: Cell membrane, caveola.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:2,000
1:50
1:50-1:200
Uniprot #: SwissProt: Q8WTV0 Human | Q61009 Mouse | P97943 Rat
Alternative names: CD36 and LIMPII analogous 1 CD36 CD36 Antigen like 1 CD36 antigen-like 1 CD36L1 CLA 1 CLA-1 CLA1 Collagen type I receptor HDLQTL6 MGC138242 SCARB1 Scavebger Receptor Class B Member 1 Scavenger receptor class B member 1 Scavenger Receptor Class B Type 1 SCRB1_HUMAN SR BI SR-BI SRB1 SRBI Thrombospondin receptor like 1 thrombospondin receptor-like 1
Images
ET1602-32_1.jpg Fig1: Western blot analysis of Scavenging Receptor SR-BI on different lysates with Rabbit anti-Scavenging Receptor SR-BI antibody (ET1602-32) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: Human liver tissue lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Rat liver tissue lysate (20 µg/Lane)

Predicted band size: 61 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-32) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1602-32_2.jpg Fig2: ICC staining of Scavenging Receptor SR-BI in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-32_3.jpg Fig3: ICC staining of Scavenging Receptor SR-BI in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-32_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-32_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-32_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.