Anti-Histone H2A(acetyl K9) antibody [SR4-15]
cat.: ET1602-34
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SR4-15
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Histone H2A aa aa 1-50 / 130 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH).
Positive control: Different cell lysates, Hela, A549, MCF-7, human tonsil tissue, human colon tissue, mouse testis tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:1,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P04908 Human | Q93077 Human | Q99878 Human | P10812 Mouse | P22752 Mouse | P02262 Rat
Alternative names: H2A H2A1B_HUMAN H2AFM HIST1H2A HIST1H2AE Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m
Images
ET1602-34_1.jpg Fig1: Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution.
Positive control:
Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
Lane 2: Untreated HeLa whole cell lysates
ET1602-34_2.jpg Fig2: ICC staining of Histone H2A(acetyl K9) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-34_3.jpg Fig3: ICC staining of Histone H2A(acetyl K9) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-34_4.jpg Fig4: ICC staining of Histone H2A(acetyl K9) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-34_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(acetyl K9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-34_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2A(acetyl K9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-34_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.