Histone H2A (hydroxyl Y39) Recombinant Rabbit Monoclonal Antibody [SR4-17]
cat.: ET1602-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SR4-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Histone H2A aa 1-83 / 130 (hydroxyl Y39) conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Positive control: | HeLa cell lysate, A549 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, human colon tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Chromosome, Nucleosome core, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1:4,000 |
| Uniprot #: | SwissProt: P04908 Human | P28001 Human | Q93077 Human | P35065 Human | Q99878 Human | C0HKE6 Mouse | C0HKE1 Mouse | P02262 Rat |
| Alternative names: | H2A/m H2A1B_HUMAN HIST1H2AE Histone H2A type 1-B/E Histone H2A.2 Histone H2A.m Histone H2A/a Histone H2A/m |
Images
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Fig1:
Western blot analysis of Histone H2A (hydroxyl Y39) on different lysates with Rabbit anti-Histone H2A (hydroxyl Y39) antibody (ET1602-35) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse thymus tissue lysate (20 µg/Lane) Lane 4: Rat thymus tissue lysate (20 µg/Lane) Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-35) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Histone H2A (hydroxyl Y39) antibody (ET1602-35) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-35) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H2A (hydroxyl Y39) antibody (ET1602-35) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-35) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H2A (hydroxyl Y39) antibody (ET1602-35) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-35) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.