Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IP |
Clonality: | Monoclonal |
Clone number: | SR40-09 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 53 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human p53 aa 343-392 / 392 (acetyl K370). |
Positive control: | HepG2 cell lysates, MCF-7, PANC-1, HepG2. |
Subcellular location: | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IP |
1:1,000 1:50 1:50 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P04637 Human | P02340 Mouse | P10361 Rat |
Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Fig1:
Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 2: HepG2 untreated whole cell lysates |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of PANC-1 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4: ICC staining of p53 (acetyl K370) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig5:
Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution. Lane 1: HeLa untreated whole cell lysates Lane 2: HeLa treated with Trichostatin A 500 ng/mL for 4 hours whole cell lysates Lane 3: NIH/3T3 untreated whole cell lysates Lane 4: NIH/3T3 treated with Trichostatin A 500 ng/mL for 4 hours whole cell lysates Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1000 dilution was used in 5% NFDM/TBST at 4°overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50000 dilution was used for 1 hour at room temperature. |