p53 (acetyl K370) Recombinant Rabbit Monoclonal Antibody [SR40-09]
cat.: ET1602-38
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: SR40-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human p53 aa 343-392 / 392 (acetyl K370).
Positive control: HepG2 cell lysates, MCF-7, PANC-1, HepG2.
Subcellular location: Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP

1:1,000
1:50
1:50
Use at an assay dependent concentration.
Uniprot #: SwissProt: P04637 Human | P02340 Mouse | P10361 Rat
Alternative names: Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53
Images
ET1602-38_1.jpg Fig1: Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates
Lane 2: HepG2 untreated whole cell lysates
ET1602-38_2.jpg Fig2: Immunocytochemistry analysis of MCF-7 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1602-38_3.jpg Fig3: Immunocytochemistry analysis of PANC-1 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1602-38_4.jpg Fig4: ICC staining of p53 (acetyl K370) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-38_5.png Fig5: Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution.

Lane 1: HeLa untreated whole cell lysates
Lane 2: HeLa treated with Trichostatin A 500 ng/mL for 4 hours whole cell lysates
Lane 3: NIH/3T3 untreated whole cell lysates
Lane 4: NIH/3T3 treated with Trichostatin A 500 ng/mL for 4 hours whole cell lysates

Lysates/proteins at 20 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 4 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1000 dilution was used in 5% NFDM/TBST at 4°overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.