p53 (acetyl K370) Recombinant Rabbit Monoclonal Antibody [SR40-09]
cat.: ET1602-38
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SR40-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human p53 aa 343-392 / 392 (acetyl K370). |
| Positive control: | HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 treated with 500ng/mL TSA for 4 hours cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, MCF-7, PANC-1, HeLa cells treated with 500ng/mL TSA for 4 hours. |
| Subcellular location: | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP FC |
1:1,000 1:50 1:50 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: P04637 Human | P02340 Mouse | P10361 Rat |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1:
Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500ng/mL TSA for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 4 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: C6 treated with 1μM TSA for 18 hours cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of PANC-1 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of HeLa cells treated with 500ng/mL TSA for 4 hours labeling p53 (acetyl K370). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-38, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.