Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody [SR31-07]
cat.: ET1602-40
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, ChIP, CUT&Tag-seq
Clonality: Monoclonal
Clone number: SR31-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 11 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5).
Positive control: CRC cell lysate, Hela cell lysate, SH-SY5Y cell lysate, Hela, CRC, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse brain tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
  ChIP
1:1,000-1:5,000
1:50-1:400
1:50-1:400
1:50-1:1,000
Use at an assay dependent concentration.
Use 0.5~2 μg for 25 μg of chromatin.
Uniprot #: SwissProt: P62805 Human | P62806 Mouse | P62804 Rat
Alternative names: Histone gene cluster 1, H4A Histone gene cluster 2, H4 dJ160A22.1 dJ160A22.2 dJ221C16.1 dJ221C16.9 FO108 H4 H4 histone family, member A H4 histone family, member B H4 histone family, member C H4 histone family, member D H4 histone family, member E H4 histone family, member G H4 histone family, member H H4 histone family, member I H4 histone family, member J H4 histone family, member K H4 histone family, member M H4 histone family, member N H4 histone, family 2 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4/p H4_HUMAN H4F2 H4F2iii H4F2iv H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK HIST1 cluster, H4A HIST1 cluster, H4B HIST1 cluster, H4D HIST2H4 Hist4 cluster, H4 Hist4h4 histone 1, H4a histone 1, H4c histone 1, H4d histone 1, H4f histone 1, H4h histone 1, H4i histone 1, H4j histone 1, H4k histone 1, H4l histone 2, H4a histone 2, H4b ......
Images
ET1602-40_1.jpg Fig1: Western blot analysis of Histone H4 (acetyl K5) on different lysates with Rabbit anti-Histone H4 (acetyl K5) antibody (ET1602-40) at 1/1,000 dilution.

Lane 1: CRC cell lysate
Lane 2: Hela cell lysate
Lane 3: SH-SY5Y cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-40) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1602-40_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody (ET1602-40) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody (ET1602-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1602-40_3.jpg Fig3: ICC staining of Histone H4 (acetyl K5) in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-40_4.jpg Fig4: ICC staining of Histone H4 (acetyl K5) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-40_5.jpg Fig5: Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H4(acetyl K5) antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1602-40 at 1/300 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
ET1602-40_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-40, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-40_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-40, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-40_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-40, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-40_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-40_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-40_11.jpg Fig11: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H4 (acetyl K5) (ET1602-40) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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