Survivin Recombinant Rabbit Monoclonal Antibody [SR44-02]
cat.: ET1602-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SR44-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Full length recombinant protein within mouse Survivin. |
| Positive control: | Rat spleen tissue, mouse testis tissue, mouse colon tissue, F9 cell lysates, L929 cell lysates. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome, Midbody. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O70201 Mouse | Q9JHY7 Rat |
| Alternative names: | API4 Apoptosis inhibitor 4 Apoptosis inhibitor survivin Apoptosis inhibitor4 Baculoviral IAP repeat containing 5 Baculoviral IAP repeat containing protein 5 Baculoviral IAP repeat-containing protein 5 BIRC 5 BIRC5 BIRC5_HUMAN EPR 1 IAP4 Survivin variant 3 alpha SVV TIAP |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Survivin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Survivin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Survivin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Western blot analysis of Survivin on F9 cell lysate with Rabbit anti-Survivin antibody (ET1602-43) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-43, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
|
Fig5:
Western blot analysis of Survivin on L929 cell lysate with Rabbit anti-Survivin antibody (ET1602-43) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-43, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.