Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]
cat.: ET1602-44
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Firefly |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SR03-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Firefly Luciferase aa 132-243 / 550. |
| Positive control: | Luciferase recombinant protein. |
| Subcellular location: | Peroxisome. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P08659 Firefly |
| Alternative names: | ec 1.13.12.7 Firefly Luciferase Luciferin 4 monooxygenase |
Images
|
Fig1:
Western blot analysis of Firefly Luciferase on Firefly Luciferase Protein with Rabbit anti-Firefly Luciferase antibody (ET1602-44) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-44, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 13 kDa Observed band size: 13 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.