Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]
cat.: ET1602-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SR41-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Escherichia coli MBP aa 331-380 / 396. |
| Positive control: | Recombinant MBP-tag protein. |
| Recommended Dilutions:
WB IP |
1:1,000 2-5 µg/ml. |
| Uniprot #: | SwissProt: P0AEX9 EscherichiaColi | P0AEY0 EscherichiaColi |
| Alternative names: | ECK4026 JW3994 Mal E MalE malJ Maltodextrin binding protein Maltose ABC transporter periplasmic protein Maltose binding periplasmic protein MMBP Periplasmic maltose binding protein |
Images
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Fig1:
MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with ET1602-46 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1602-46 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 60 mins at room temperature. Lane 1: MBP Tag fusion protein lysate (input). Lane 2: ET1602-46 IP in MBP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
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Fig2:
Western blot analysis of Maltose Binding Protein on MBP-tag only cell lysate with Rabbit anti-Maltose Binding Protein antibody (ET1602-46) at 1/1,000 dilution. Lysates/proteins at 5 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1602-46, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 43 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.