Cleaved Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR01-02]
cat.: ET1602-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Pig |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SR01-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Caspase-3 aa 28-67 / 277. |
| Positive control: | Camptothecin (2 μM) treated Jurkat cell lysate, Hela, PC-3M, human colon carcinoma tissue, human placenta tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P42574 Human |
| Alternative names: | Caspase3 A830040C14Rik Apopain CASP 3 CASP-3 CASP3 CASP3_HUMAN Casp3a Caspase 3 Caspase 3, apoptosis-related cysteine peptidase Caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis-related cysteine protease a Caspase-3 subunit p12 Caspase3 CC3 CPP 32 CPP-32 CPP32 CPP32B Cysteine protease CPP32 EC 3.4.22.56 ICE3 LICE mldy OTTHUMP00000165052 OTTHUMP00000165053 OTTHUMP00000165054 PARP cleavage protease Procaspase3 Protein Yama SCA 1 SCA-1 SCA1 SREBP cleavage activity 1 Yama Yama protein |
Images
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Fig1:
Western blot analysis of Cleaved Caspase-3 on different lysates with Rabbit anti-Cleaved Caspase-3 antibody (ET1602-47) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25μM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25μM Etoposide for 16 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-47) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cleaved Caspase-3 antibody (ET1602-47) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Cleaved Caspase-3 antibody (ET1602-47) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.