Anti-Active Caspase-3 antibody [SR01-02]
cat.: ET1602-47
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Pig
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SR01-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Caspase-3 aa 20-60.
Positive control: Camptothecin (2 μM) treated Jurkat cell lysate, Hela, PC-3M, human tonsil tissue, human spleen tissue.
Subcellular location: Cytoplasm
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P42574 Human
Alternative names: A830040C14Rik antibody Apopain antibody CASP 3 antibody CASP-3 antibody CASP3 antibody CASP3_HUMAN antibody Casp3a antibody Caspase 3 antibody Caspase 3, apoptosis-related cysteine peptidase antibody Caspase 3, apoptosis-related cysteine protease antibody Caspase 3, apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody Caspase3 antibody CC3 antibody CPP 32 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody ICE3 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SCA1 antibody SREBP cleavage activity 1 antibody Yama antibody Yama protein antibody
Images
ET1602-47_1.jpg Fig1: Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Camptothecin (2 μM) treated Jurkat cell lysate
Lane 2: Untreated Jurkat cell lysate
ET1602-47_2.jpg Fig2: ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-47_3.jpg Fig3: ICC staining of Active Caspase-3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1602-47_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Active Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1602-47_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Active Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.