ET1602-48

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IF-Tissue, ChIP
Clonality:	Monoclonal
Clone number:	SR04-20

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 15 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within N-terminal human Histone H3.

Positive control:

Hela cell lysate, NIH/3T3 cell lysate, MCF-7.

Subcellular location:

Nucleus, Chromosome.

Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  ChIP

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Use 0.5~2 μg for 25 μg of chromatin.

Uniprot #:

Alternative names:

HIST1 cluster, H3J Histone gene cluster 1, H3 histone family, member E Histone gene cluster 1, H3G H3 histone family, member J HIST1 cluster, H3E HIST1 cluster, H3I Histone gene cluster 1, H3C FLJ92264 H3 histone family, member A H3 histone family, member B H3 histone family, member C H3 histone family, member D H3 histone family, member F H3 histone family, member H H3 histone family, member I H3 histone family, member K H3 histone family, member L H3 histone family, member T H3 histone, family 3A H3.1 H3.3A H3/a H3/b H3/c H3/d h3/f H3/h H3/i H3/j H3/k H3/l H3/t H31_HUMAN H3F1K H3F3 H3F3A H3FA H3FB H3FC H3FD H3FF H3FH H3FI H3FJ H3FK H3FL HIST1 cluster, H3A HIST1 cluster, H3B HIST1 cluster, H3C HIST1 cluster, H3D HIST1 cluster, H3F HIST1 cluster, H3G HIST1 cluster, H3H HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST......

	Fig1: Western blot analysis of Histone H3 (mono methyl K36) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate
	Fig2: ICC staining Histone H3(mono methyl K36) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (mono methyl K36) (ET1602-48) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.