M6PR Recombinant Rabbit Monoclonal Antibody [SR45-09]
cat.: ET1602-5
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SR45-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 274 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human M6PR aa 2,442-2,491 / 2,491. |
| Positive control: | SiHa cell lysate, human skin tissue lysate, HeLa, human tonsil tissue. |
| Subcellular location: | Golgi apparatus membrane, Endosome membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:10,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P11717 Human | Q07113 Mouse Entrez Gene: 25151 Rat |
| Alternative names: | 300 kDa mannose 6 phosphate receptor 300 kDa mannose 6-phosphate receptor Cation independent mannose 6 phosphate receptor Cation-independent mannose-6-phosphate receptor CD222 CD222 antigen CI Man 6 P receptor CI Man-6-P receptor CI MPR CI-M6PR CI-MPR CIMPR IGF 2 receptor IGF 2R IGF II receptor IGF-II receptor IGF2 receptor Igf2r Insulin like growth factor 2 receptor Insulin like growth factor II receptor Insulin-like growth factor 2 receptor Insulin-like growth factor II receptor M6P R M6P/IGF2 receptor M6P/IGF2R M6PR mannose 6 phosphate receptor mannose 6 phosphate receptor, cation independent MPR 300 MPR300 MPRI MPRI_HUMAN |
Images
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Fig1:
Western blot analysis of M6PR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: human skin tissue lysate |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling M6PR with Rabbit anti-M6PR antibody (ET1602-5) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-M6PR antibody (ET1602-5) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-M6PR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of M6PR was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-5, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.