Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]
cat.: ET1603-1
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SZ02-01
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Caveolin-1 aa 129-178 / 178.
Positive control: A549 cell lysate, A431 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse heart tissue lysate, rat lung tissue lysate, rat heart tissue lysate, A549, MCF-7, NIH/3T3, human liver tissue, human liver carcinoma tissue, human uterus tissue, mouse heart tissue.
Subcellular location: Golgi apparatus membrane, Cell membrane, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:5,000
1:50-1:200
1:50-1:200
1:200-1:10,000
Uniprot #: SwissProt: Q03135 Human | P49817 Mouse | P41350 Rat
Alternative names: BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21
Images
ET1603-1_1.jpg Fig1: Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at different dilutions.

Lane 1/2: A549 cell lysate
Lane 3/4: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 20 kDa
Observed band size: 20 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1603-1_2.jpg Fig2: Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/1,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: A431 cell lysate (10 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)

Predicted band size: 20 kDa
Observed band size: 20/18 kDa

Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1603-1_3.jpg Fig3: ICC staining of Caveolin-1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-1_4.jpg Fig4: ICC staining of Caveolin-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-1_5.jpg Fig5: ICC staining of Caveolin-1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-1_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-1_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-1_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-1_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.