ATF2 Recombinant Rabbit Monoclonal Antibody [SZ17-01]
cat.: ET1603-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: SZ17-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ATF2 aa 456-505 / 505.
Positive control: HeLa cell lysate, 293T cell lysate, THP-1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, RAW264.7 cell lysate, SW480, Hela, MCF-7, PC-12, human breast carcinoma tissue.
Subcellular location: Nucleus, Membrane, Cytoplasm, Mitochondrion, Mitochondrion outer membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P15336 Human | P16951 Mouse | Q00969 Rat
Alternative names: Activating transcription factor 2 Activating transcription factor 2 splice variant ATF2 var2 ATF 2 Atf-2 Atf2 ATF2 protein ATF2_HUMAN cAMP Response Element Binding Protein 2 cAMP response element binding protein CRE BP1 cAMP response element-binding protein CRE-BP1 cAMP responsive element binding protein 2, formerly cAMP-dependent transcription factor ATF-2 cAMP-responsive element-binding protein 2 CRE BP1 CRE-BP CREB 2 CREB-2 CREB2 CREBP1 Cyclic AMP dependent transcription factor ATF 2 Cyclic AMP-dependent transcription factor ATF-2 Cyclic AMP-responsive element-binding protein 2 D130078H02Rik D18875 HB 16 HB16 Histone acetyltransferase ATF2 MGC105211 MGC105222 MGC111558 MGC142504 mXBP MXBP protein Tg(Gzma-Klra1)7Wum TREB 7 TREB7
Images
ET1603-15_1.jpg Fig1: Western blot analysis of ATF2 on different lysates with Rabbit anti-ATF2 antibody (ET1603-15) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: THP-1 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: RAW264.7 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 70 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1603-15_2.jpg Fig2: ICC staining of ATF2 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-15_3.jpg Fig3: ICC staining of ATF2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-15_4.jpg Fig4: ICC staining of ATF2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-15_5.jpg Fig5: ICC staining of ATF2 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-15_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.