Anti-Caspase-8 antibody [SZ01-08]
cat.: ET1603-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SZ01-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Caspase-8 aa 217-250.
Positive control: Jurkat cell lysate, K562 cell lysate, Hela, MCF-7, HepG2, human tonsil tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:5,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q14790 Human
Alternative names: ALPS2B antibody Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody Apoptotic cysteine protease antibody Apoptotic protease Mch-5 antibody Apoptotic protease Mch5 antibody CAP4 antibody CASP-8 antibody CASP8 antibody CASP8_HUMAN antibody Caspase 8 antibody Caspase 8 apoptosis related cysteine peptidase antibody Caspase-8 subunit p10 antibody CED 3 antibody FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease antibody FADD-like ICE antibody FLICE antibody FLJ17672 antibody ICE-like apoptotic protease 5 antibody MACH alpha 1/2/3 protein antibody MACH antibody MACH beta 1/2/3/4 protein antibody MCH5 antibody MGC78473 antibody MORT1 associated ced 3 homolog antibody MORT1-associated CED-3 homolog antibody OTTHUMP00000163717 antibody OTTHUMP00000163720 antibody OTTHUMP00000163724 antibody OTTHUMP00000163725 antibody OTTHUMP00000165062 antibody OTTHUMP00000165063 antibody OTTHUMP00000165064 antibody OTTHUMP00000206552 antibody OTTHUMP00000206582 antibody
Images
ET1603-16_1.jpg Fig1: Western blot analysis of Caspase-8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
ET1603-16_2.jpg Fig2: ICC staining of Caspase-8 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-16_3.jpg Fig3: ICC staining of Caspase-8 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-16_4.jpg Fig4: ICC staining of Caspase-8 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-16_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.