Phospho-c-Myc (T58 + S62) Recombinant Rabbit Monoclonal Antibody [SZ02-06]
cat.: ET1603-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SZ02-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of Human c-Myc. |
| Positive control: | HCT 116 cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, EL4 cell lysate, EL4 treated with 25μM MG-132 for 4 hours cell lysate, K562. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IP |
1:1,000-1:5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01106 Human | P01108 Mouse |
| Alternative names: | Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Class E basic helix-loop-helix protein 39 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc related translation/localization regulatory factor MYC_HUMAN Myc2 MYCC Niard Nird Proto-oncogene c-Myc Transcription factor p64 v myc avian myelocytomatosis viral oncogene homolog v myc myelocytomatosis viral oncogene homolog |
Images
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Fig1:
Western blot analysis of Phospho-c-Myc (T58 + S62) on different lysates with Rabbit anti-Phospho-c-Myc (T58 + S62) antibody (ET1603-24) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 treated with 25μM MG-132 for 4 hours cell lysate Lane 3: HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-24) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-c-Myc (T58 + S62) on different lysates with Rabbit anti-Phospho-c-Myc (T58 + S62) antibody (ET1603-24) at 1/1,000 dilution. Lane 1: EL4 cell lysate Lane 2: EL4 treated with 25μM MG-132 for 4 hours cell lysate Lane 3: EL4 treated with 25μM MG-132 for 4 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Flow cytometric analysis of Phospho-c-Myc (T58 + S62) was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1603-24, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.