Caspase-9 Recombinant Rabbit Monoclonal Antibody [SZ29-01]
cat.: ET1603-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SZ29-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Caspase-9 aa 289-338 / 416. |
| Positive control: | C2C12 cell lysate, Hela cell lysate, NIH/3T3, HepG2 cell, A549 cell, human tonsil tissue, human cervix carcinoma tissue, human colon tissue, human colon carcinoma tissue, mouse spleen tissue. |
| Subcellular location: | Mitochondrion, cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP FC |
1:500-1:5,000 1:100-1:500 1:100-1:500 1:400-1:5,000 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: P55211 Human | Q8C3Q9 Mouse |
| Alternative names: | Caspase9 APAF-3 APAF3 Apoptosis related cysteine peptidase Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP-9 CASP9 CASP9_HUMAN Caspase 9 apoptosis related cysteine peptidase Caspase 9 Dominant Negative Caspase 9c Caspase-9 Caspase-9 subunit p10 ICE LAP6 ICE like apoptotic protease 6 ICE-LAP6 ICE-like apoptotic protease 6 MCH6 PPP1R56 protein phosphatase 1, regulatory subunit 56 RNCASP9 |
Images
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Fig1:
Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C2C12 cell lysate Lane 2: Hela cell lysate |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling Caspase-9 with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-27) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Caspase-9 antibody (ET1603-27) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-27) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of NIH/3T3 cells labeling Caspase-9. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1603-27, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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