Bax Recombinant Rabbit Monoclonal Antibody [SZ3-07]
cat.: ET1603-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Goat |
| Applications: | WB, IP, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SZ3-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bax aa 1-50 / 192. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, bEnd.3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, C2C12, HeLa. |
| Subcellular location: | Mitochondrion membrane, Cytoplasm. |
| Recommended Dilutions:
WB FC IP IF-Cell |
1:20,000 1:1,000 Use at an assay dependent concentration. 1:100 |
| Uniprot #: | SwissProt: Q07812 Human | Q07813 Mouse | Q63690 Rat |
| Alternative names: | Apoptosis regulator BAX BAX Bax-protein BAX_HUMAN BAXA Baxdelta2G9 Baxdelta2G9omega Baxdelta2omega Bcl-2-like protein 4 BCL2 associated X protein BCL2 associated X protein omega BCL2 associated X protein transcript variant delta2 Bcl2-L-4 BCL2L4 membrane isoform alpha |
Images
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Fig1:
Western blot analysis of Bax on different lysates with Rabbit anti-Bax antibody (ET1603-34) at 1/20,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: bEnd.3 cell lysate Lane 5: C2C12 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-34) at 1/20,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
All lanes: Western blot analysis of Bax with anti-Bax antibody [SZ3-07] (ET1603-34) at 1/1,000 dilution. Lane 1/2: Wild-type Hela whole cell lysate (20 µg). Lane 3/4: Bax knockout Hela whole cell lysate (20 µg). ET1603-34 was shown to specifically react with Bax in wild-type Hela cells. No band was observed when Bax knockout samples were tested. Wild-type and Bax knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1603-34, 1/1,000) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of C2C12 cells labeling Bax with Rabbit anti-Bax antibody (ET1603-34) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bax antibody (ET1603-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling Bax. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1603-34, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.