Phospho-V-Myb+C-Myb (S11) Recombinant Rabbit Monoclonal Antibody [SZ04-81]
cat.: ET1603-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SZ04-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser11 of Human V-Myb+C-Myb. |
| Positive control: | AGS, human tonsil tissue, MCF-7. |
| Subcellular location: | Nucleus, cytosol. |
| Recommended Dilutions:
IF-Cell IF-Tissue IHC-P |
1:100 1:50-1:200 1:200-1:1,000 |
| Uniprot #: | SwissProt: P10242 Human |
| Alternative names: | C myb C myb protein Cmyb MYB Myb proto oncogene V myb myeloblastosis viral oncogene homolog |
Images
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Fig1:
Immunocytochemistry analysis of AGS cells labeling Phospho-V-Myb+C-Myb (S11) with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Phospho-V-Myb+C-Myb (S11) antibody (ET1603-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.